This document presents technical details, hardware and software of a complete imaging system which uses a fast CCD sensor and a 41 Msample/s A/D converter to acquire full-frame 12 bit/pixel digitised images with a time resolution of 1.25 ms/image. The noise characteristics and the photon-capture capabilities of the system were analysed in detail. At the maximum readout rate, noise from the electronics amounted to ~300e-, without cooling.
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Optical methods are interesting to physiologists and neuroscientists for several reasons. Of particular importance is the capability of recording simultaneously from multiple sites and the possibility to reach fast sampling rates. Therefore it is fundamental to develop imaging systems that, combining both features, achieve a high spatio-temporal resolution. In this respect, CCD sensors are well suited for detecting low-light level signals from fluorescent probes introduced into living cells. These devices possess high quantum efficiency for converting light into electrical signals that allow monitoring variations in the intracellular concentration of Ca2+ and other fundamental ions within the cell cytoplasm. Here we provide a detailed description of an imaging system based on a sensitive CCD sensor with high readout rate (16 MHz). Sensor’s output was digitised at 12 bit/pixel by customised electronics permitting image acquisition rates up to 800 frames/s with a full-frame resolution of 128´ 128 pixels.
This project fits more for custom made battery casing that can have more space to hide things inside. This switch with few alterations can be used for any other project as well but is designed based on e-bike needs! This project use a clever functionality of....
The circuit described here uses ultrasonic oscillations and operates based on the propagation velocity of these oscillations in the air. Thus, we can easily determine the distance of two points if the time within which the wave travels this distance is....
Sometimes the precious metals are hidden too deep and are not detected except with complicated devices. In many cases, however, small pieces of precious metal buried near the surface can be detected by relatively simple means. Everyone is very attractive to....
Many times for various reasons we forget or can not water the plants that we have in our homes. And many humidity sensors units just notify us with a beeping sound or with a flashing light, that the pot needs watering. But what if we are away from home? This....
The principle behind a metal detector is really very simple. Proof of this is the circuit that follows, in which it proves that the construction of a metal detector can be done in the minute, with few components that we find very easily everywhere. With the....
The sub-harmonic bass generator is a sound producing unit for guitars. The sound it produces looks very much like that of the bass guitar. The octave generator is sometimes quite unusual since it does not produce any of the usual ways of coloring the sound,....
In the past the sounding (sea bottom measurement) was done with the "bullet", that is, with a heavy lead object that the seamen plucked into the sea hung from a calibrated rope. As soon as the "bullet" reached the bottom, the depth appeared directly from the....
This Amplifier was designed to have the following specifications: Distortion less than 0.1% at full power of 100W even at 20KHz. Power has to be attributed to an extended bandwidth. The output transistors must be protected against short circuits. The power....
A series of LEDs serve to alert the gardener when plants need water. Using two conventional digital integrated LEDs and a series of LEDs, we make a very useful device for gardening. The device detects the amount of water in the pot and alerts the grower.....
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